Academia.edu no longer supports Internet Explorer.
To browse Academia.edu and the wider internet faster and more securely, please take a few seconds to upgrade your browser.
Interspecific hybridization is an important method to widen variations and to develop novel cultivars. Interspecific cross-pollination was carried out among three species of Cucurbita (C. moschata, C. ficifolia and C. martinezii) as male parents with ten cultivars of C. pepo as female parents. Only four cultivars of C. pepo (Eskandarani cv., Queen F , Jedida F and MHTC77 F ) were successful 1 1 1 in producing interspecific hybrids with C. moschata. Traditional sexual hybridization technique was unsuccessful in producing hybrids between C. ficifolia and C. pepo as well as C. martinezii and C. pepo. However, among the ten cultivars of C. pepo, only Queen F1 with C. ficifolia and MHTC77 F1 with C. martinezii were able to give fertile set fruits but failed to produce seeds. Therefore, hybridity was accomplished when embryo rescue technique was employed. The immature embryos of C. ficifolia × C. pepo (Queen F1) and C. martinezii × C. pepo (MHTC77 F1) recorded regeneration efficiency of 40% and 15%, respectively.
Homozygous plants can be obtained in a single generation using doubled haploid technology, which can tremendously facilitate breeding programs. The present study reports on in vitro regeneration and production of double haploid plants through anther and ovule cultures resulting from the hybridization of cultivated species of Cucurbita pepo L. with three other Cucurbita species i.e. C. moschata L., C. ficifolia and C. martinezii L. Among six sucrose-2,4-Dichlorophenoxyacetic acid (2,4-D) combinations tested, the addition of 90 g l-1 sucrose and 1 mg l-1 2,4-D into the medium was found to be more suitable for callus regeneration from anthers. Anthers from the hybrid C. pepo L. (Queen F1) × C. moschata L. regenerated the highest percentage (40%) of plantlets from callus and highest average number of plantlets per callus (7.5) when the culture medium was supplemented with the aforementioned concentrations of sucrose and 2,4-D. Ovules collected from the hybrid C. pepo L. (MHTC77 F1) × C. moschata L. produced the highest percentage of regenerated plantlets (25.33%), the highest percentage of responded ovules (18.67%) and the highest average number of plantlets per ovule (3.37). Cold pretreatment had negative effects on gynogenesis with the exception of the hybrid C. pepo L. (MHTC77 F1) × C. moschata L. Chromosome counting in the root tips of androgenic plantlets revealed 50% of haploid and (n = x = 20) and 50% of dihaploid plantlets (2n = 2x = 40). The assessment of root tips from gynogenic plantlets resulted in 60% haploid (2n = x = 20) and 40% diploid (2n = 2x = 40) plants. The androgenic strains were evaluated for their vegetative and reproductive traits compared with open local cultivar C. pepo L. (Eskandarani cv.). While the majority of regenerated strains were intermediate between the cultivated species of C. pepo L. and other Cucurbita species, two regenerated strains proved superior to the local cultivar 'Eskandarani' in number of fruits (early and total yield/plot) as well as in total fruit yield per plot.
HortScience
Toward Introgression of Ornamental Cucurbita Germplasm: Generation of C. maxima ×pepo hybrids2004 •
Cucurbita maxima and C. pepo are difficult to hybridize, and it was our objective to generate F1 hybrids between ornamental cultivars of the two species. C. maxima `Lakota' and C. pepo `Jack O'Lantern'; and `OZ'; were selected as parents. `Lakota' (L) is an heirloom, hubbard-type cultivar producing pear-shaped, red-orange fruit with dark green mottling, `Jack O'Lantern'; (J) is an open-pollinated Halloween-type pumpkin cultivar and `OZ' is a Halloween-type hybrid. Sixteen plants of each cultivar were greenhouse-grown in a CRB design during the period July-Sept. 2003. Interspecific crosses were made in both directions, with intraspecific crosses (J × O) and selfs (L) serving as controls. Fruits were harvested about 20 d after pollination. Embryos were excised under aseptic conditions and grown on either full strength Murashige and Skoog (MS) media with 6% sucrose (S6), full strength MS media with 6% maltose (M6), or half strength MS media with 3% sucro...
2019 •
An intraspecific hybridization programme among Cucumis species involving eight genetically diverse parents of C. melo L. varietal group viz., IC-0599709, Punjab Sunehri, Pusa Madhuras, Kashi Madhu (C. melo L.), AHK-119 (C. melo var. callosus), AHLM-2 (C. melo var. utilissimus), AHS-82 (C. melo var. momordica) and Arya-1 (C. melo var. chate) were undertaken. Among the dessertic F1 hybrids, IC-0599709 × Punjab Sunehri resulted best with respect to first fruit harvest (73.33 days), fruit weight (938.00 g), fruit diameter (11.90 cm), flesh thickness (3.07 cm), total soluble solids (9.73%) and flesh colour (salmon orange). F2 population of AHK-119 × Kashi Madhu produced maximum number of marketable fruits per plant (11.2) weighing 350.00 g in 86.1 days. It was also found to be tolerant to high temperature. The F2 population of AHK-119 × Kashi Madhu were found to be tolerant to high temperature. The existing variability among the parents, intra-specific crosses and segregating generations...
Bangladesh Journal of Botany
Development and characterization of intraspecific hybrids derived from Cucumis melo LAn intraspecific hybridization programme among Cucumis species involving eight genetically diverse parents of C. melo L. varietal group viz., IC-0599709, Punjab Sunehri, Pusa Madhuras, Kashi Madhu (C. melo L.), AHK-119 (C. melo var. callosus), AHLM-2 (C. melo var. utilissimus), AHS-82 (C. melo var. momordica) and Arya-1 (C. melo var. chate) were undertaken. Among the dessertic F1 hybrids, IC-0599709 × Punjab Sunehri resulted best with respect to first fruit harvest (73.33 days), fruit weight (938.00 g), fruit diameter (11.90 cm), flesh thickness (3.07 cm), total soluble solids (9.73%) and flesh colour (salmon orange). F2 population of AHK-119 × Kashi Madhu produced maximum number of marketable fruits per plant (11.2) weighing 350.00 g in 86.1 days. It was also found to be tolerant to high temperature. The F2 population of AHK-119 × Kashi Madhu were found to be tolerant to high temperature. The existing variability among the parents, intra-specific crosses and segregating generations...
Journal of Applied Research on Medicinal and Aromatic Plants
Evaluation of combining ability in Cucurbita pepo L. and Cucurbita moschata Duchesne accessions for fruit and seed quantitative traits2018 •
Plant Cell Tissue and Organ Culture
A method for increased plant regeneration from immature F1 and BC1 embryos of Cucurbita maxima Duch. x C. pepo L. hybrids1989 •
Plants have been regenerated from abnormal embryos with spongy cotyledons and albino sectors, derived from Cucurbita maxima and C. pepo F1 and BC1 hybrids. Shoot regeneration was induced directly from the cotyledons without an intervening callus phase on the medium without hormones. On the rooting medium, shoots continued to proliferate, which allowed for further multiplication in vitro. The number of plants obtained varied with genotype and ranged up to 65 plants per embryo.
Journal of Applied Research on Medicinal and Aromatic Plants
Genetic diversity of Cucurbita pepo L. and Cucurbita moschata Duchesne accessions using fruit and seed quantitative traits2018 •
2018 •
In order to estimate the mean heterosis, mid parent heterosis and heterobeltiosis (HB), three diallel crossings of Cucurbita moschata were evaluated, each formed by six parents with three levels of inbreeding (S0, S1, S2). A randomized complete block experimental design was used with four replicates, arranged in split plots. The variables yield per plant (YPP) and average fruit weight (AFW) were analyzed. The hybrids between S1 or S2 inbred lines presented heterotic superiority regarding to those between S0 parents for the variables YPP and AFW. Likewise, the hybrids between S2 inbred lines reported heterotic superiority in comparison to those among S1 inbred lines, for such variables. The hybrids between S2 inbred lines that reported the highest expression levels of HB for YPP were P1xP3, P2xP6 and P1xP2, with values ranging between 131.42 and 98.24%; while the hybrids among S1 inbred lines that recorded the highest values of HB, for this same variable, were P3xP5 and P1xP5 with va...
Facta universitatis - series: Electronics and Energetics
On VHDL synthesis of self-checking two-level combinational circuits2004 •
Indonesian Journal on Geoscience
Nanggulan Formation and Its Problem As a Basement in Kulonprogo Basin, Yogyakarta2010 •
Journal of Hydro-environment Research
Effect of sinusoidal corrugated bed on hydraulic jump characteristics2009 •
International Journal of Food Microbiology
Insights from genome-wide approaches to identify variants associated to phenotypes at pan-genome scale: Application to L. monocytogenes' ability to grow in cold conditions2018 •
IET Electric Power Applications
Redundant switching state based DTC for six‐phase symmetrical induction motor driven by nine‐switch inverter2019 •
2006 •
The Bulletin of Legal Medicine
Aesthetic Concerns in Forensic Expertise-Two Cases2015 •
2018 •
Ethik in der Medizin
Kommentar II zum Fall: „Mutmaßlicher Widerruf einer Patientenverfügung?“2010 •
2020 •
2017 •
2020 •
2002 •
Journal of Biological Chemistry
Periodate Modification of Human Serum Transferrin Fe(III)-binding Sites1995 •
The Journal of neuroscience : the official journal of the Society for Neuroscience
Pharmacogenetic excitation of dorsomedial prefrontal cortex restores fear prediction error2015 •
arXiv (Cornell University)
Trillion Dollar Words: A New Financial Dataset, Task & Market Analysis2023 •
Pamukkale University Journal of Engineering Sciences
Sentiment analysis with Twitter2016 •
Uluslararası Deniz ve Kıyı Folkloru Sempozyumu Bildiri Kitabı
Doğu Karadeniz’de Unutulan Bir Balıkçılık Türü Irmakçılık (Havyarcılık) ve “Gemiciler Kalkalım” Türküsü / A Forgotten Type of Fishing in the Eastern Black Sea Region: River Fishing (Caviar Fishing) and the Folk Song “Gemiciler Kalkalım”2023 •
Journal of Youth Development
Neighborhood Youth Centers and Families as Supportive Environments for Youth in High Risk Urban Settings2009 •