High-performance liquid chromatography/NMR spectrometry/mass spectrometry: further advances in hyphenated technology

J Mass Spectrom. 1997 Jan;32(1):64-70. doi: 10.1002/(SICI)1096-9888(199701)32:1<64::AID-JMS450>3.0.CO;2-7.

Abstract

The earlier use of combined liquid chromatography/NMR spectrometry/mass spectrometry (LC/NMR/MS) involved the use of a particle beam interface. This paper describes further developments of this hyphenated technology, in particular the incorporation of an electrospray interface into the LC/NMR/MS system. This improved LC/NMR/MS system was designed for the support of a combinatorial library program. The power of this technique is demonstrated in the successful structural elucidation of each compound in a mixture of commercially available peptides.

MeSH terms

  • Chemical Phenomena
  • Chemistry
  • Chromatography, High Pressure Liquid* / methods
  • Magnetic Resonance Spectroscopy / methods
  • Mass Spectrometry* / methods
  • Peptides / analysis
  • Peptides / chemistry*
  • Spectrophotometry

Substances

  • Peptides