Genetic analysis suggests there were at least two migrations into the Americas.
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Perego, U. A. et al. Curr. Biol. doi:10.1016/j.cub.2008.11.058 (2008).
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Ledford, H. Earliest Americans took two paths. Nature (2009). https://doi.org/10.1038/news.2009.7
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DOI: https://doi.org/10.1038/news.2009.7